Modernization of protein purification and characterization equipment for advanced structure function studies

Proteins are the workhorses of living cells, and fulfill a variety of essential functions. State-of-the art understanding of protein function requires, in additional to biochemical in vitro and in vivo studies, a detailed characterization of the protein structure and dynamics. This knowledge can be achieved with advanced scattering, diffraction and spectroscopic techniques, which however require extremely high quality protein samples. The major part of the Carlsberg grant will contribute to new equipment for in situ dynamic light scattering (DLS) which will allow monitoring of sample quality, stability and aggregation in preparation of advanced structural studies and also help in several other projects where macromolecular aggregation and assembly needs to be monitored.