A 3D Holo-Tomographic Live Cell Imaging Microscope for Biophotonic and Photobiological Studies of Cells and Tissues

Name of applicant

Michael Kühl

Institution

University of Copenhagen

Amount

DKK 1,107,709

Year

2021

Type of grant

Research Infrastructure

Summary

A holotomographic imaging system enabling non-invasive, label-free 3D imaging of cell structure and refractive index via quantitative phase imaging in combination with traditional fluorescence readout will be established. The microscope will enable quantification and mapping of bio-optical properties in photosynthetic microorganisms and tissues of organisms containing phototrophic symbionts, which is essential for a more quantitative understanding of their photobiology and light propagation in complex living structures

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