Novel microfluidic platform for investigation of virus-biofilm interactions
Navn på bevillingshaver
Juliane Fjelrad Christfort
Institution
Université Côte d Azur
Beløb
DKK 700,000
År
2021
Bevillingstype
Internationalisation Fellowships
Hvad?
This research project aims to establish a physiological in vitro cell model to study the interactions between human herpes viruses (HHVs) and pathogenic bacteria involved in the progression of periodontal disease (PD), and use it to propose new treatment strategies. As a major novelty, we will use a compact platform (tabletop, laptop-sized format), based on centrifugal microfluidics or Lab-on-a-Disc (LoD), for cell culturing in flow (perfusion). The microfluidic disc, where the cells are cultured, has the size and shape of a CD, is compatible with conventional microscopy techniques, and can be combined with a custom-designed, miniaturized microscope for real-time, online monitoring.
Hvorfor?
PD is the most common chronic inflammatory disease in humans. In PD, bacterial inflammations in the structures supporting the teeth (gingiva, periodontal ligament and alveolar bone) can lead to tooth loss and contribute to systemic inflammation. The disease is difficult to treat with antibiotics, and often complex and expensive surgeries are needed. Traditionally, PD has been ascribed to the overgrowth of pathogenic bacteria, but the bacteria alone do not adequately explain the clinical characteristics of the disease. Recently, HHVs have been suggested as a possible trigger for oral dysbiosis resulting in progression of PD. However, there is an urgent need for in vitro platforms that can facilitate studies of the interactions between bacteria and viruses, to propose new treatments.
Hvordan?
We will design, optimize and realize a user-friendly, pump- and tubing-free LoD platform to study the cross-talk between HHVs and periodontal bacteria (e.g. Enterococcus faecalis and Streptococcus salivarius). To do so, we will grow virus-infected oral cells under flow in the presence of bacteria, and observe the cellular response by PCR-based detection of viral markers, release of cytokines and oxidative stress. Additionally, we will use the LoD platform to screen the effect of antiviral drugs, with and without co-delivery of antibiotics, as a novel treatment strategy for PD.